Elzbieta Urasinska (former name Bedner), MD, PhD

Department of Pathology, Pomeranian Medical University
Unii Lubelskiej St. 1, 71-252 Szczecin, Poland
Phone/fax: +48 91 4870032

Education

Career/Employment

Honours, Awards, Fellowships, Membership of Professional Societies Awards

1997, 1998 A. Jurzykowski Fellowship awarded by Alfred Jurzykowski Foundation, New York, NY

Membership in Scientific Organizations

Polish Society of Pathology
International Society for Analytical Cytology

Selected publications (last five years)

1. Masiuk M, Urasinska E, Domagala W.: Simultaneous measurement of nucleolin and estrogen receptor in breast cancer cells by laser scanning cytometry. Anticancer Res. 2004; 24(2B): 963-6.
2. Mazur L, Augustynek A, Deptala A, Halicka HD, Bedner E.: Effects of WR-2721 and cyclophosphamide on the cell cycle phase specificity of apoptosis in mouse bone marrow. Anticancer Drugs. 2002; 13(7):751-8.
3. Darzynkiewicz Z, Bedner E, Smolewski P, Lee BW, Johnson GL.: Detection of caspases activation in situ by fluorochrome-labeled inhibitors of caspases (FLICA). Methods Mol Biol. 2002; 203:289-99.
4. Darzynkiewicz Z, Bedner E, Smolewski P.: In situ detection of DNA strand breaks in analysis of apoptosis by flow- and laser-scanning cytometry. Methods Mol Biol. 2002; 203:69-77.
5. Darzynkiewicz Z, Smolewski P, Bedner E.: Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death. Clin Lab Med. 2001; 21(4):857-73.
6. Chen S, Ruan Q, Bedner E, Deptala A, Wang X, Hsieh TC, Traganos F, Darzynkiewicz Z.: Effects of the flavonoid baicalin and its metabolite baicalein on androgen receptor expression, cell cycle progression and apoptosis of prostate cancer cell lines. Cell Prolif. 2001; 34(5):293-304.
7. Du L, Smolewski P, Bedner E, Traganos F, Darzynkiewicz Z.: Selective protection of mitogenically stimulated human lymphocytes but not leukemic cells from cytosine arabinoside-induced apoptosis by LY294002, a phosphoinositol-3 kinase inhibitor. Int J Oncol. 2001; 19(4):811-9.
8. Smolewski P, Bedner E, Gorczyca W, Darzynkiewicz Z.:"Liquidless" cell staining by dye diffusion from gels and analysis by laser scanning cytometry: potential application at microgravity conditions in space. Cytometry. 2001; 44(4):355-60.
9. Smolewski P, Bedner E, Du L, Hsieh TC, Wu JM, Phelps DJ, Darzynkiewicz Z.: Detection of caspases activation by fluorochrome-labeled inhibitors: Multiparameter analysis by laser scanning cytometry. Cytometry. 2001; 44(1):73-82.
10. Darzynkiewicz Z, Bedner E, Smolewski P.: Flow cytometry in analysis of cell cycle and apoptosis. Semin Hematol. 2001; 38(2):179-93.
11. Bedner E, Du L, Traganos F, Darzynkiewicz Z.: Caffeine dissociates complexes between DNA and intercalating dyes: application for bleaching fluorochrome-stained cells for their subsequent restaining and analysis by laser scanning cytometry. Cytometry. 2001; 43(1):38-45.
12. Deptala A, Bedner E, Darzynkiewicz Z.: Unique analytical capabilities of laser scanning cytometry (LSC) that complement flow cytometry. Folia Histochem Cytobiol. 2001; 39(2):87-9.
13. Darzynkiewicz Z, Li X, Bedner E.: Use of flow and laser-scanning cytometry in analysis of cell death. Methods Cell Biol. 2001;66:69-109.
14. Gorczyca W, Deptala A, Bedner E, Li X, Melamed MR, Darzynkiewicz Z.: Analysis of human tumors by laser scanning cytometry. Methods Cell Biol. 2001; 64:421-43.
15. Darzynkiewicz Z, Bedner E, Traganos F. Difficulties and pitfalls in analysis of apoptosis. Methods Cell Biol. 2001; 63:527-46.
16. Halicka HD, Bedner E, Darzynkiewicz Z.: Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis. Exp Cell Res. 2000; 260(2):248-56.
17. Bedner E, Li X, Kunicki J, Darzynkiewicz Z.: Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry. Cytometry. 2000; 41(2):83-8.
18. Bedner E, Smolewski P, Amstad P, Darzynkiewicz Z. Activation of caspases measured in situ by binding of fluorochrome-labeled inhibitors of caspases (FLICA): correlation with DNA fragmentation. Exp Cell Res. 2000;259(1): 308-13.
19. Bedner E, Ruan Q, Chen S, Kamentsky LA, Darzynkiewicz Z.: Multiparameter analysis of progeny of individual cells by laser scanning cytometry. Cytometry. 2000; 40(4):271-9.
20. Lin CW, Darzynkiewicz Z, Li X, Traganos F, Bedner E, Tse-Dinh YC. Differential expression of human topoisomerase IIIalpha during the cell cycle progression in HL-60 leukemia cells and human peripheral blood lymphocytes. Exp Cell Res. 2000; 256(1):225-36.
21. Darzynkiewicz Z, Bedner E.: Analysis of apoptotic cells by flow and laser scanning cytometry. Methods Enzymol. 2000; 322:18-39.
22. Deptala A, Li X, Bedner E, Cheng W, Traganos F, Darzynkiewicz Z.: Differences in induction of p53, p21WAF1 and apoptosis in relation to cell cycle phase of MCF-7 cells treated with camptothecin. Int J Oncol. 1999; 15(5):861-71.
23. Darzynkiewicz Z, Bedner E, Li X, Gorczyca W, Melamed MR.: Laser-scanning cytometry: A new instrumentation with many applications. Exp Cell Res. 1999; 249(1):1-12.
24. Bedner E, Halicka HD, Cheng W, Salomon T, Deptala A, Gorczyca W, Melamed MR, Darzynkiewicz Z.: High affinity binding of fluorescein isothiocyanate to eosinophils detected by laser scanning cytometry: a potential source of error in analysis of blood samples utilizing fluorescein-conjugated reagents in flow cytometry. Cytometry. 1999; 36(1):77-82.
25. Bedner E, Li X, Gorczyca W, Melamed MR, Darzynkiewicz Z.: Analysis of apoptosis by laser scanning cytometry. Cytometry. 1999; 35(3):181-95.
26. Darzynkiewicz Z, Li X, Bedner E.: Detection of DNA strand breakage in the analysis of apoptosis and cell proliferation by flow and laser scanning cytometry. Methods Mol Biol. 1999; 113:607-19.